Four different simple, accurate, rapid, economical spectroscopic methods namely Zero order, First derivative, Area Under Curve and Three wavelength spectroscopy have been developed and validated for estimation of Ondansetron in tablet dosage form using methanol as a solvent according to ICH Guidelines Q2R1. Absorbance of each solution was measured at 303.80 nm for zero order spectrophotometry. First derivative spectra are obtained by transformations at Δλ of 4 nm and scaling factor 10 and absorbance measured at 240.20 nm. Peak area is measured between 256.40-278.20 nm for AUC method. For three wavelength spectroscopy, absorbance was recorded at three wavelengths λ1= 255.60 nm, λ2 = 266.60 nm, λ3 = 277.60 nm. Ondansetron show linearity over the range of 4-20 μg/ml for all developed methods. Statistical comparisons by one way ANOVA shows all methods have equal applicability for estimation of Ondansetron in tablet dosage forms.
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